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The Silene Transcriptome

One of the most exciting opportunities created by recent advances in DNA sequencing technology is the ability to rapidly generate genome-wide data for previously uncharacterized genetic systems. In particular, deep sequencing of cDNA libraries (i.e., transcriptome or EST sequencing) is an efficient method for providing broad coverage of the expressed gene content within a genome.

Members of the angiosperm genus Silene are widely used in studies of ecology and evolution. However, the availability of genomic resources for this group has remained limited. In an attempt to bolster these resources, we have used 454 transcriptome sequencing and de novo assembly to analyze gene expression at a genome-wide level in Silene vulgaris. The primary goals of this project are two-fold: 1) to identify and sequence a major fraction of coding genes in this species and 2) to generate a genome-wide set of variable markers to be used in population genetic analyses.

This project was planned and overseen by members of the Taylor Lab at the University of Virginia Department of Biology. It was carried out in conjunction with genome sequencing centers at both Indiana University and the University of Virginia. This research was supported by the National Science Foundation.